Figure EV5. RNF34 is required for the clearance of damaged mitochondria upon viral infection.

1. Immunoblot showing MAVS, IRF3‐p, LC3, TOM20, and HSP60 levels in siCtrl‐ or siRNF34‐1‐transfected cells infected with VSV for the indicated times. α‐Tubulin was used as a loading control.
2. Immunoblot showing the levels of activated caspase‐3 in 293T cells infected with VSV for the indicated times and cultured in the presence or absence of Z‐VAD (50 μM). α‐Tubulin was used as a loading control.
3. Representative confocal images of immunofluorescence staining for TOM20 clearance in shCtrl‐ or shRNF34‐1 cells infected with VSV and stained with anti‐T0M20 and anti‐LAMP1 antibodies. Red represents TOM20; green represents LAMP1. Scale bar, 10 μm.
4. Quantification of TOM20 colocalization with LAMP1 in the cells shown in (C).
5. The product of the mean fluorescence intensity of TOM20 in GFP‐RNF34‐transfected cells and the mean fluorescence intensity of GFP‐transfected cells.
6. Representative confocal images of TOM20 immunofluorescence staining in cells transfected with GFP or GFP‐RNF34. Scale bar, 10 μm.

Data information: Cell‐based studies were performed independently at least three times with comparable results.Source data are available online for this figure.