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. 2019 May 31;38(14):e101260. doi: 10.15252/embj.2018101260

Figure 1. iNKT TN mice have increased numbers of tissue‐resident iNKT cells.

Figure 1

  1. Diagram representing the placement of various tissues analyzed for iNKT cells. mLN = mesenteric lymph node; sdLN = skin‐draining lymph node.
  2. Relative iNKT cell yield in various tissues from TN mouse lines compared to C57BL/6 mouse lines. Tissues were isolated from indicated C57BL/6 or iNKT TN mouse lines and stained with anti‐CD3 and CD1d‐(PBS57)‐tetramer.
  3. Spleen, mLN, sdLN, liver, adipose, and lung lymphocytes from Jα18−/− or Vα14 mice were stimulated in vitro with RAW‐CD1d cells and 1 μg α‐GalCer. An additional sample of Vα14 lymphocytes from each organ was plated with RAW‐CD1d cells but no α‐GalCer. Supernatants were collected after 24 h and cytokine concentration determined by cytokine bead array. Error bars are SD of mean values from three different mice per group. Results shown are representative of two independent experiments where n = 3 biological replicates.