Figure 2. iNKT cells from TN and C57BL/6 mice show similar influence of tissue microenvironment on NKT1, NKT2, and NKT17 subsets.

• A–F
  Lymphocytes from the indicated tissues of C57BL/6 and Vα14 mice were stained with anti‐CD3 and CD1d‐(PBS57)‐tetramer, before they were fixed, permeabilized, and stained with antibodies to T‐bet, RORγt, and PLZF. Results shown are gated on CD3⁺CD1d‐tetramer⁺ cells.
• G
  The percentage of CD3⁺ CD1d‐tetramer⁺ iNKT cells in each organ that stained positively for PLZF, T‐bet, and RORγt are shown. **P < 0.01, Mann–Whitney test. Error bars are SD.

Data information: Results shown are representative of three independent experiments where n = 3 biological replicates.