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. 2019 May 31;38(14):e101260. doi: 10.15252/embj.2018101260

Figure EV3. Magnitude of regulation of IgG1+ B cells by NKT cells varies by mouse facility, but is dependent on CD1d.

Figure EV3

  1. Stool collected from C57BL/6 and CD1d−/− mice was analyzed by ELISA for IgG1 as described in Fig 3. Mice were age‐ and sex‐matched and housed in the Longwood Center facility. C57BL/6 n = 18; CD1d−/− n = 5. Mann–Whitney test. Error bars are SD.
  2. Spleen, mLN, and PP cells were harvested from wild‐type or CD1d−/− mice, stained with antibodies to B220 and IgG1, and analyzed by flow cytometry. Mice were age‐ and sex‐matched and housed in the Longwood Center facility. C57BL/6 n = 5; CD1d−/− n = 3. Mann–Whitney test. Error bars are SD.
  3. Analysis was performed identically to that shown in Fig 3F, except that mice were housed in the Smith Building at Dana‐Farber Cancer Institute prior to moving to the Longwood Center at Dana‐Farber Cancer Institute. Results in Fig 3 are entirely from mice housed in the Longwood Center facility. C57BL/6 n = 10; Vα14−/− n = 12; Jα18−/− n = 3. Mann–Whitney test. Error bars are SD.
  4. Culture supernatants from Fig 5D and E were measured by ELISA for IgM. np = not performed. Mann–Whitney test. Error bars are SD.