Anti‐ovalbumin antibodies were detected by ELISA from serum of immunized mice as indicated. Secondary antibodies to either IgG1 or IgA were used for detection.
Fecal pellets were collected from immunized mice and diluted in water at 10 mg stool/ml. Anti‐ovalbumin antibodies were detected by ELISA as indicated. Secondary antibodies to pan‐Ig, IgG1, or IgA were used for detection.
Data information: Error bars are SD. Mice used were Jα18
−/− (
n = 10 total), iNKT transnuclear (
n = 7 total), or littermate controls of iNKT TN mice that did not inherit the Vα14 allele (B6,
n = 12 total).
