Figure EV5. Adoptively transferred iNKT cells do not accumulate in Peyer's patches and do not produce IL‐4.

1. Jα18^−/− mice were lightly irradiated with 100 Gy prior to receiving either no cell transfer (bottom panels) or transfer of 10 million pooled spleen and LN cells from Va14 TN mice. Input cells were verified by flow cytometry to contain > 30% CD1d‐tetramer⁺ cells. Two weeks post‐transfer, the indicated tissues were harvested and analyzed by flow cytometry. Representative of n = 5 recipient mice per group. Representative of three independent experiments.
2. Lymphocyte preparations from the indicated tissues of Jα18^−/− mice from (A) were cocultured with RAWd cells and 1 μg α‐GalCer as in Fig 1. IL‐4 was measured by ELISA of 48‐h culture supernatants. Error bars are SD of triplicate samples.