Skip to main content
. 2019 May 31;38(14):e101260. doi: 10.15252/embj.2018101260

Figure 6. Oral vaccination with α‐GalCer leads to specific antibody production in NKT cell proficient mice.

Figure 6

  • A
    Mice were administered ovalbumin by oral gavage, with or without α‐GalCer at the indicated time points, at the indicated amounts. Mice used were Jα18−/− (n = 10 total), iNKT transnuclear (n = 7 total), or littermate controls of iNKT TN mice that did not inherit the Vα14 allele (B6, n = 12 total).
  • B, C
    OVA‐specific antibody titers from feces and blood were determined by ELISA from the mice in (A). Shown are optical density values for samples collected on Day 52 post‐vaccination (7 days after the final boost). Error bars are SD.
  • D
    Cells from spleen, mesenteric lymph node, and Peyer's patches of Vα14 TN mice were permeabilized, fixed, and stained with the indicated antibodies. Plots shown are gated on CD1d‐(PBS57)‐tetramer+ CD3+ iNKT cells.
  • E
    Quantification of data from (D), n = 3 mice per group. Mann–Whitney test. Error bars are SEM. *P < 0.05.