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. 2019 May 31;38(14):e101260. doi: 10.15252/embj.2018101260

Figure 7. PP‐iNKT cells exhibit a unique gene expression profile compared to spleen iNKT and PP CD4+ cells.

Figure 7

  1. Principal component analysis of RNAseq samples from biological replicates of PP‐iNKT, spleen iNKT, and PP CD4+ cells
  2. Differential expression analysis comparing transcriptomes of spleen iNKT cells versus PP‐iNKT cells.
  3. Heatmap of FPKM values from genes uniquely upregulated in PP‐iNKT cells as compared to both spleen iNKT and PP CD4 cells. Red indicates higher expression, and blue indicates lower expression.
  4. Spleen and PP cells from Vα14 TN mice were stained with the indicated antibodies, analyzed by flow cytometry, and gated on CD1d‐(PBS57)‐tetramer+ CD3+ cells.
  5. Quantification of data from (D), n = 11 mice per group. Mann–Whitney test. Error bars are SEM.