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Induction of HIF-1α by H. pylori in the presence of AHA or urea in gastric epithelial cells. AGS cells were infected with H. pylori 26695 at MOI 100 for 8 h. Protein extracts were prepared and HIF-1α and β-actin protein levels were analyzed by Western blotting. Experiments were performed in the presence/absence of (A) the urease inhibitor AHA 20 mM or (B) urea 2 mM. Deferoxamine (5 µM) was included as a positive control for HIF-1 induction in normoxic conditions.
