Table 1.
Glycolipids identified by HILIC LC–MS and MS/MS in F. vesiculosus (mass error < 5 ppm). MGDG and DGDG molecular species were identified as [M + NH4]+ ions while SQDG and SQMG were identified as [M – H]− ions. Numbers in parentheses (C:N) indicate the number of carbon atoms (C) and double bonds (N) in the fatty acid side chains.
| Galactolipids [M + NH4]+ | ||
| Observed m/z | Lipid Species (C:N) | Fatty Acyl Chain |
| Monogalactosyl diacylglycerol - MGDG | ||
| 746.5765 | MGDG (32:1) | 18:1/14:0, 16:1/16:0 |
| 768.5621 | MGDG (34:4) | 18:4/16:0, 18:3/16:1 |
| 770.5764 | MGDG (34:3) | 18:3/16:0 |
| 772.5935 | MGDG (34:2) | 18:2/16:0, 18:1/16:1 |
| 774.6074 | MGDG (34:1) | 18:1/16:0 |
| 788.5307 | MGDG (36:8) | 18:4/18:4 |
| 790.5459 | MGDG (36:7) | 18:3/18:4 |
| 792.5614 | MGDG (36:6) | 18:3/18:3 |
| 794.5772 | MGDG (36:5) | 18:2/18:3, 20:5/16:0 |
| 796.5929 | MGDG (36:4) | 20:4/16:0, 18:2/18:2 |
| 814.5449 | MGDG (38:9) | 20:5/18:4 |
| 816.5608 | MGDG (38:8) | 20:5/18:3, 20:4/18:4 |
| 818.5766 | MGDG (38:7) | 20:5/18:2, 20:4/18:3 |
| 820.5926 | MGDG (38:6) | 20:5/18:1, 20:4/18:2 |
| 822.6073 | MGDG (38:5) | 20:4/18:1 |
| 824.6235 | MGDG (38:4) | 20:4/18:0 |
| 842.5751 | MGDG (40:9) | 20:5/20:4 |
| 844.594 | MGDG (40:8) | 20:4/20:4 |
| Monogalactosyl monoacylglycerol - MGMG | ||
| 536.3795 | MGMG (18:1) | |
| 530.3329 | MGMG (18:4) | |
| 532.3488 | MGMG (18:3) | |
| 534.3642 | MGMG (18:2) | |
| Digalactosyl diacylglycerol - DGDG | ||
| 906.6148 | DGDG (32:2) | 14:0/18:2 |
| 908.6305 | DGDG (32:1) | 14:0/18:1, 16:0/16:1 |
| 930.6135 | DGDG (34:4) | * |
| 932.6305 | DGDG (34:3) | 18:3/16:0 |
| 934.6461 | DGDG (34:2) | 18:2/16:0, 18:1/16:1 |
| 936.6618 | DGDG (34:1) | 18:1/16:0 |
| 938.678 | DGDG (34:0) | 18:0/16:0 |
| 952.5992 | DGDG (36:7) | 18:3/18:4 |
| 954.6148 | DGDG (36:6) | 18:3/18:3 |
| 956.6305 | DGDG (36:5) | 20:5/16:0, 18:2/18:3 |
| 958.6461 | DGDG (36:4) | 20:4/16:0, 18:2/18:2 |
| 960.6623 | DGDG (36:3) | 18:1/18:2 |
| 962.678 | DGDG (36:2) | 18:1/18:1 |
| 964.6936 | DGDG (36:1) | 18:1/18:0 |
| 976. 5997 | DGDG (38:9) | 20:5/18:4 |
| 978.6144 | DGDG (38:8) | 20:5/18:3, 20:4/18:4 |
| 980.6289 | DGDG (38:7) | 20:4/18:3, 20:5/18:2 |
| 982.6448 | DGDG (38:6) | 20:4/18:2, 20:5/18:1 |
| 984.6594 | DGDG (38:5) | 20:4/18:1 |
| 986.6767 | DGDG (38:4) | 20:4/18:0 |
| Digalactosyl monoacylglycerol - DGMG | ||
| 672.417 | DGMG (16:0) | |
| 692.3857 | DGMG (18:4) | |
| 694.4014 | DGMG (18:3) | |
| 696.417 | DGMG (18:2) | |
| 698.4327 | DGMG (18:1) | |
| Sulfolipids [M – H]– | ||
| Sulfoquinovosyl monoacylglycerol - SQMG | ||
| 527.2515 | SQMG (14:0) | |
| 553.2664 | SQMG (16:1) | |
| 555.2829 | SQMG (16:0) | |
| 577.2673 | SQMG (18:3) | |
| 579.2832 | SQMG (18:2) | |
| 581.2984 | SQMG (18:1) | |
| Sulfoquinovosyl diacylglycerol - SQDG | ||
| 737.4495 | SQDG (28:0) | 14:0/14:0 |
| 763.4648 | SQDG (30:1) | 16:0/14:1 |
| 765.4804 | SQDG (30:0) | 16:0/14:0 |
| 787.4646 | SQDG (32:3) | 18:3/14:0 |
| 789.4804 | SQDG (32:2) | 18:2/14:0 |
| 791.4958 | SQDG (32:1) | 18:1/14:0 |
| 793.5099 | SQDG (32:0) | 18:0/14:0 |
| 813.4803 | SQDG (34:4) | 18:3/16:1 |
| 815.4958 | SQDG (34:3) | 18:3/16:0 |
| 817.5107 | SQDG (34:2) | 18:2/16:0 |
| 819.5269 | SQDG (34:1) | 18:1/16:0 |
| 839.4977 | SQDG (36:5) | 20:5/16:0 |
| 841.5106 | SQDG (36:4) | 20:4/16:0 |