Table 2.
Phospholipid identified by HILIC LC–MS and MS/MS in F. vesiculosus (mass error < 5 ppm). PC and LPC molecular species were identified as [M + H]+ ions while PG, LPG, PI, LPI, PE, and LPE were identified as [M – H]– ions. Numbers in parentheses (C:N) indicates the number of carbon atoms (C) and double bonds (N) in the fatty acid side chains.
| Phospholipids [M + H]+ | ||
| Observed m/z | Lipid species (C:N) | Fatty acyl chain |
| Phosphatidylcholine - PC | ||
| 730.538 | PC (32:2) | 16:1/16:1 |
| 756.55 | PC (34:3) | 16:1/18:2 |
| 758.5689 | PC (34:2) | 16:0/18:2, 16:1/18:1 |
| 760.5835 | PC (34:1) | |
| 780.553 | PC (36:5) | 18:2/18:3 |
| 784.585 | PC (36:3) | 18:1/18:2 |
| 786.6005 | PC (36:2) | 18:1/18:1 |
| 804.5545 | PC (38:7) | |
| 806.5683 | PC (38:6) | |
| 808.5831 | PC (38:5) | |
| Lyso-phosphatidylcholine - LPC | ||
| 494.3241 | LPC (16:1) | |
| 496.3392 | LPC (16:0) | |
| 522.3556 | LPC (18:1) | |
| Phospholipids [M − H]− | ||
| Observed m/z | Lipid species (C:N) | Fatty acyl chain |
| Phosphatidylglycerol - PG | ||
| 717.471 | PG (32:2) | 16:0/16:2, 16:1/16:1 |
| 719.4868 | PG (32:1) | 16:1/16:0, 14:0/18:1 |
| 721.5033 | PG (32:0) | 16:0/16:0, 14:0/18:0 |
| 741.4713 | PG (34:4) | 16:1/18:3 |
| 743.4862 | PG (34:3) | 16:0/18:3, 16:1/18:2 |
| 745.5018 | PG (34:2) | 16:0/18:2 |
| 747.5183 | PG (34:1) | 16:1/18:0 |
| 749.5324 | PG (34:0) | 16:0/18:0 |
| 769.4987 | PG (36:4) | 16:0/20:4, 18:2/18:2 |
| 771.516 | PG (36:3) | 18:1/18:2 |
| 773.5321 | PG (36:2) | 18:1/18:1 |
| Lyso-phosphatidylglycerol - LPG | ||
| 481.2569 | LPG (16:1) | |
| 483.2733 | LPG (16:0) | |
| 509.2887 | LPG (18:1) | |
| Phosphatidylinositol - PI | ||
| 831.4984 | PI (34:3) | 16:0/18:3 |
| 833.5175 | PI (34:2) | 16:0/18:2 |
| 835.5339 | PI (34:1) | 16:0/18:1 |
| 837.5491 | PI (34:0) | 16:0/18:0 |
| 857.5196 | PI (36:4) | 16:0/20:4 |
| 859.5352 | PI (36:3) | 18:0/18:3 |
| 861.5505 | PI (36:2) | 18:0/18:2, 18:1/18:1 |
| 863.5674 | PI (36:1) | 18:0/18:1 |
| Lyso-Phosphatidylinositol - LPI | ||
| 597.306 | LPI (18:1) | |
| Phosphatidylethanolamine | ||
| 686.4758 | PE (32:2) | 14:0/18:2, 16:1/16:1 |
| 714.5067 | PE (34:2) | 16:1/18:1 |
| 716.5227 | PE (34:1) | 16:0/18:1 |
| 762.5073 | PE (38:6) | 18:2/20:4 |
| 764.5232 | PE (38:5) | 18:1/20:4 |
| 784.4918 | PE (40:9) | 20:4/20:5 |
| 786.5076 | PE (40:8) | 20:4/20:4 |
| Lyso-Phosphatidylethanolamine - LPE | ||
| 498.2613 | LPE (20:5) | |
| 500.2779 | LPE (20:4) | |