Table 3.

Betaine lipids identified by HILIC LC–MS and MS/MS in F. vesiculosus (mass error < 5 ppm). DGTS, MGTS, DGTA, and MGTA molecular species were identified as [M + H]⁺ ions. Numbers in parentheses (C:N) indicate the number of carbon atoms (C) and double bonds (N) in the fatty acid side chains, ** with the contribution of sodium adducts.

Betaine lipids [M + H]+
Observed m/z	Lipid species (C:N)	Fatty acyl chain
Diacylglyceryl trimethyl-homoserine - DGTS
656.5468	DGTS (28:0)	14:0/14:0
682.5628	DGTS (30:1)	14:0/16:1
684.5787	DGTS (30:0)	14:0/16:0
704.5458	DGTS (32:4)	16:0/16:4
706.5617	DGTS (32:3)	14:0/18:3, 16:0/16:3
708.5778	DGTS (32:2)	16:0/16:2
710.5933	DGTS (32:1)	14:0/18:1, 16:0/16:1
732.5774	DGTS (34:4)	16:0/18:4
734.5932	DGTS (34:3)	16:0/18:3
736.6083	DGTS (34:2)	16:0/18:2
738.624	DGTS (34:1)	16:0/18:1
762.6248	DGTS (36:3)	18:0/18:3
764.6405	DGTS (36:2)	18:0/18:2
Monoacylglyceryl trimethyl-homoserine - MGTS
446.347	MGTS (14:0)
472.3632	MGTS (16:1)
474.3792	MGTS (16:0)
494.3477	MGTS (18:4)
496.3634	MGTS (18:3)
498.3796	MGTS (18:2)
500.3943	MGTS (18:1)
Diacylglyceryl hydroxymethyl-trimethyl-β-alanine - DGTA
656.547	DGTA (28:0)	14:0/14:0
680.5433	DGTA (30:2)
682.5623	DGTA (30:1)	14:0/16:1
684.5782	DGTA (30:0)	14:0/16:0
704.5464	DGTA (32:4)	14:0/18:4
706.5625	DGTA (32:3)	14:0/18:3
708.5778	DGTA (32:2)	14:0/18:2
710.5933	DGTA (32:1)	14:0/18:1, 16:0/16:1
732.5776	DGTA (34:4)	**14:0/20:4
734.5935	DGTA (34:3)	14:0/20:3, 16:0/18:3
736.6091	DGTA (34:2)	14:0/20:2, 16:0/18:2
738.6241	DGTA (34:1)	16:0/18:1
758.5925	DGTA (36:5)	16:0/20:5
760.6085	DGTA (36:4)	**16:0/20:4
762.6246	DGTA (36:3)	16:0/20:3
764.6406	DGTA (36:2)	18:1/18:1
784.6084	DGTA (38:6)	18:2/20:4
786.6235	DGTA (38:5)	18:1/20:4
Monoacylglyceryl hydroxymethyl-trimethyl-β-alanine - MGTA
446.3464	MGTA (14:0)
472.3618	MGTA (16:1)
474.3777	MGTA (16:0)
494.3463	MGTA (18:4)
496.3623	MGTA (18:3)
498.3778	MGTA (18:2)
500.3941	MGTA (18:1)
520.3619	MGTA (20:5)
522.3779	MGTA (20:4)
524.3958	MGTA (20:3)