Figure 1.

Effects of hydrolysis pH (A), temperature (B), substrate concentration (C), pepsin dosage (D), reaction time (E), and glutamate concentration (F) on free amino acids reduction of the plastein products. Unless otherwise noted, temperate was 40 °C, pH was 5.0, substrate concentration was 40%, pepsin dosage was 500 U/g, reaction time was 3 h, and l-[1-¹³C]glutamate concentration was 10 mg/mL. Each point is shown as the means ± standard deviation (SD) (n = 3). Different letters indicate significant differences (p < 0.05).