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Steps involved in a SERS experiments based on an immunoassay. Reproduced with permission from Porter et al. [425]. (a) The substrate is functionalized with a capture antibody; (b) The SERS tag is synthesized by assembling a plasmonic core, a Raman reporter and a detection antibody; (c) In the assay procedure, the antigen (analyte) is sandwiched in between the SERS tag and the substrate. Copyright (2008), the Royal Society of Chemistry.
