Different up- and down-regulated proteins when the boundary formed compared to the non-boundary samples based on coverage value. Cold shock proteins (Csps) were more than 10-fold up-regulated compared to both control samples. Other proteins such as patatin-like phosphate, Dof-B protein (DofB), tetratricopeptide repeat (TPR) and chaperonin GroEL1 were also significantly up-regulated in the boundary. Porins, putative outer membrane proteins P1, and TonB-dependent receptor were down-regulated significantly in the case of the boundary when compared with both control samples, DK1622-DK1622 and ΔMXAN_0049–ΔMXAN_0049.