Table 1.
Primers used for RT–PCR analysis.
| Genes | Primer Sequence (5′→3′) | Annealing Temperature(°C) | Product Size (bp) | References |
|---|---|---|---|---|
| SOX17 | F: GGCGCAGCAGAATCCAGA | 65 | 61 | [47] |
| R: CCACGACTTGCCCAGCAT | ||||
| CXCR4 | F: ACTACACCGAGGAAATGGGCT | 60 | 133 | NM_003467.2 This study |
| R: CCCACAATGCCAGTTAAGAAGA | ||||
| GATA6 | F: CCATGACTCCAACTTCCACC | 62 | 214 | [52] |
| R: ACGGAGGACGTGACTTCGGC | ||||
| AFP | F: CTTTGGGCTGCTCGCTATGA | 60 | 131 | NM_001134 This study |
| R: GCATGTTGATTTAACAAGCTGCT | ||||
| HNF3β | F: CCTACTCGTACATCTCGCTCATC | 65 | 69 | [42] |
| R: CGCTCAGCGTCAGCATCTT | ||||
| CK18 | F: TCGCAAATACTGTGGACAATGC | 60 | 171 | NM_199187.1 This study |
| R: GCAGTCGTGTGATATTGGTGT | ||||
| ALB | F: GCACAGAATCCTTGGTGAACAG | 65 | 101 | [53] |
| R: ATGGAAGGTGAATGTTTCAGCA | ||||
| G6P | F: TCAGCTCAGGTGGTCCTCTT | 62 | 291 | [54] |
| R: CCTCCTTAGGCAGCCTTCTT | ||||
| C/EBPα | F: ACAAGAACAGCAACGAGTACCG | 65 | 129 | [53] |
| R: CATTGTCACTGGTCAGCTCCA | ||||
| CYP2B6 | F: GTGATCTTTTGTGTCTGGTTGC | 62 | 138 | NM_000767.4 This study |
| R: GATAGACGGAAGCAGTAGGAAG | ||||
| HDAC1 | F: CCAAGTACCACAGTGATGACTACATT | 65 | 135 | NM_004964.2 This study |
| R: AGAACTCAAACAGGCCATCAAA | ||||
| GAPDH | F: TGCACCACCAACTGCTTAGC | 60 | 87 | [53] |
| R: GGCATGGACTGTGGTCATGAG | ||||
| β-ACTIN | F: TCACCACCACGGCCGAGCG | 60 | 350 | X00351 This study |
| R: TCTCCTTCTGCATCCTGTCG |