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. 2019 Jun 14;8(6):588. doi: 10.3390/cells8060588

Figure 4.

Figure 4

Knockdown of TIRAP expression suppressed the proliferation of non-small cell lung cancer cells. (A) Western blot analysis of TIRAP expressions in H1975, H1650, and LTEP-a2 cells after transfection with Neg. and TIRAP siRNA. All cells were exposed to Neg. and TIRAP siRNA for 12 h, followed by culturing with complete medium for 48 h before protein extraction and Western analysis. (B) qRT-PCR analysis of TIRAP expressions in H1975, H1650, and LTEP-a2 cells after transfection with Neg. and TIRAP siRNA. All cells were exposed to Neg. and TIRAP siRNA for 12 h, followed by culturing with complete medium for 36 h before RNA extraction and qRT-PCR analysis. (C) Cell proliferation assay of H1975, H1650, and LTEP-a2 cells after transfection with Neg. and TIRAP siRNA. All cells were exposed to Neg. and TIRAP siRNA for 12 h, followed by dissociation with trypsin and transferring into 96-well plates for proliferation analysis. (D) Cell colony formation assay of H1975, H1650, and LTEP-a2 cells after transfection with Neg. and TIRAP siRNA. All cells were exposed to Neg. and TIRAP siRNA for 12 h, followed by dissociation with trypsin and transferring into 6-well plates for colony formation analysis. (E) Cell cycle analysis of H1975, H1650, and LTEP-a2 cells after transfection with Neg. and TIRAP siRNA. All cells were exposed to Neg. and TIRAP siRNA for 12 h, followed by culturing with complete RPMI-1640 media for 36 h before cell cycle analysis. TIRAP, Toll/interleukin 1 receptor domain-containing adaptor protein. Neg. siRNA, negative control siRNA. Bars represent mean ± SD. *, p < 0.05; **, p < 0.01.