Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 11;8(6):574. doi: 10.3390/cells8060574

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Evolution of selenoprotein mRNA levels in response to the CRISPR-Cas9-VLP treatments and/or addition of selenium (100 nM) in Hek293 and HAP1 cell lines. (A) The RNA extracts used in Figure 6 were also used for RT-qPCR analysis to measure selenoprotein and housekeeping mRNA levels. The geometrical mean of five housekeeping genes (Hpcb, Rps13, rRNA 18S, Hrpt, and Gapdh) was used to normalize mRNA abundance. Selenoprotein mRNA levels are represented in logarithmic scale in a heatmap. The values are given in Table S2 and Table S3. (B) To validate the stability of steady state levels of selenoprotein mRNAs, the values obtained in control conditions (no VLP, no added Se) were plotted as a function of values obtained with 100 nM Se (top histograms) or with tRNA VLP treated conditions (bottom histograms) in a logarithmic scale. For clarity reasons, only mRNAs for which a minimum of two-fold difference (and p-value below 0.05) were represented in yellow circle and labeled in the graphs.