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Rpn15 mediates the association of Rpn3 with Rpn7. siRNA targeting Rpn7, Rpn3, and Rpn6 was performed using HEK293T cells stably expressing Rpn15-GFP. Accumulated intermediates were collected and immunoprecipitated with anti-GFP antibody. When Rpn7 was knocked down, only Rpn3 was coimmunoprecipitated with Rpn15. None of the lid subunits was identified when Rpn3 was knocked down. Intermediates of Rpn3-7-15 were detected when Rpn6 was knocked down.
