Figure 1.

PITG_15718.2 is upregulated in sporangia and during the early stages of infection. The hyphae were scraped with a glass rod from the plants on which P. infestans grew at 18 °C for two weeks. The sporangia were collected from the suspension, then incubated on ice and stored at 4 °C for 3 h to release the zoospores. The PITG_15718 expression level was assessed by qRT-PCR at 0, 12, 24, 48, 72 and 96 h after inoculation of P. infestans. Error bars represent SD of the mean values of three biological replicates. The experiment was repeated three times with similar results.