Figure 2.

Validation of positive transgenic lines of PITG_15718.2 potato (Désirée). (a) PCR detected 18 transgenic individuals using PITG_15718.2-specific primers with agarose gel electrophoresis. A total of 13 positive lines out of 18 lines were obtained; (b) detection of the expression level of PITG_15718.2 using semiquantitative RT-PCR for 13 positive lines. The StEF gene was the endogenous control. PITG_15718.2 was highly expressed in lines 7, 8, 9 and 15; (c) western blot showing that PITG_15718.2 was highly accumulated in lines 7, 8, 9 and 15. Total protein extracts from S. tuberosum (Désirée and lines 7, 8, 9 and 15). HA-tagged fusion proteins were detected by probing western blots with HA antibodies.