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. 2019 Jun 21;20(12):3031. doi: 10.3390/ijms20123031

Figure 3.

Figure 3

Constitutive expression of PITG_15718.2 enhanced susceptibility to P. infestans in potato. (a) Three PITG_15718.2-expressing lines were challenged with P. infestans HLJ. Images were photographed at four days post-inoculation (dpi). Désirée was used as control; (b) the lesion size was significantly enhanced on both transgenic potato lines compared to the control. The disease lesion diameter was calculated from at least 15 leaves using the average of the longest and shortest diameters on each leaf. * indicates significant (t test, p < 0.05) differences, ** indicates extremely significant (t test, p < 0.01) differences; (c) determination of P. infestans biomass by quantitative real-time PCR. P. infestans–specific primers referred the PI-O8 element were used for PCR with the mixed-DNA template that was isolated from infected leaves (containing the DNA of P. infestans and S. tuberosum). The host gene (StEF) was used to normalize the results. ** indicates extremely significant (t test, p < 0.01) differences. The experiment was repeated three times with similar results.