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. 2019 Jun 21;20(12):3030. doi: 10.3390/ijms20123030

Figure 2.

Figure 2

Effects of RGE on changes in mitochondrial dynamics caused by Aβ accumulation in the subiculum of 5XFAD mice. (A) Immunohistochemical staining for translocases of mitochondrial outer membrane 20 (Tom20) was performed to evaluate the mitochondrial dynamics (scale bar = 20 μm). (B) The quantification results of Tom20 fluorescence intensity were normalized to the values for vehicle-treated wild-type (WT) mice. (C) Tom20 (+) area was quantified and plotted as a percentage. Total fragmentation (D), small fragmentation (E), continuous structures (F), and average Tom20 particle size (G) were quantified by setting the immunoreactivity area of Tom20 to a threshold by mitochondrial structure size to identify the fusion and fission of mitochondria. Data are presented as mean ± SEM. ** p < 0.01 and *** p < 0.001: vehicle-treated WT mice (white bar) versus vehicle-treated 5XFAD mice (black bar). # p < 0.05 and ## p < 0.01: vehicle-treated 5XFAD mice versus Aβ + RGE-treated 5XFAD mice (red bar).