Figure 12.

Human HEK293 cells or cells engineered to express Sec61α alleles bearing indicated point mutations were treated with increasing concentrations of ipomoeassin F (A) or mycolactone (B), and cell viability was assayed at 72 h with the Alamar Blue assay (mean ± SD; n = 4 technical quadruplicates). Parental or HCT116 cells containing mutations in Sec61A1 were seeded at 3 × 10⁴/well in 96-well plates in triplicate. Ipomoeassin F (C) or mycolactone (D) were added at various concentrations with the carrier DMSO used as a control. Cell viability was measured at 96 h with the Alamar Blue assay (mean ± SEM of n = 3 independent experiments performed in triplicate).