Table 1.

List of primers used for site-directed mutagenesis. Mismatched nucleotides are marked with red letters.

Q93H _FW	5’ GATCGATTCTGACTATCATGGCCAGTTGATGATTTCC 3’
Q93H_REV	5’ GGAAATCATCAACTGGCCATGATAGTCAGAATCGATC 3’
Q93R_fw	5’ GACTATCGGGGCCAGTTGATGATTTCCGTGTGG 3’
Q93R_rev	5’ CTGGCCCCGATAGTCAGAATCGATCAATCCTACCAGG 3’
E114D_fw	5’ CATTCAACCTGGCGATCGCATCGCCCAG 3’
E114D_rev	5’ CTGGGCGATGCGATCGCCAGGTTGAATG 3’
R115K_fw	5’ CCTGGCGAAAAAATCGCCCAGATGATTTTTGTTCCGGTAGTACAGGCTGAATTTAATCTGGTGG 3’
R115K_rev	5’ CTGGGCGATTTTTTCGCCAGGTTGAATGGTGAAGCTGTCCTGACCACGG 3’
ER114-5DK_fw	5’ CAACCTGGCGATAAAATCGCCCAGATGATTTTTGTTCCGGTAGTACAGGCTGAATTTAATCTGGTGG 3’
ER114-5DK_rev	5’ CATCTGGGCGATTTTATCGCCAGGTTGAATGGTGAAGCTGTCCTGACCACGG 3’
F145W_fw	5’ GAAGGCGGCTGGGGTCACTCTGGTCGTCAGTAACACATACGGATCCGGC 3’
F145W_rev	5’ AGAGTGACCCCAGCCGCCTTCACCGCGGTCGGTGGCGTC 3’
H147S_fw	5’ CCGCGGTGAAGGCGGCTTTGGTAGCTCTGGTCGTCAGTAACAC 3’
H147S_rev	5’ GTGTTACTGACGACCAGAGCTACCAAAGCCGCCTTCACCGCGG 3’