Skip to main content
. 2019 Jun 21;8(6):625. doi: 10.3390/cells8060625

Figure 4.

Figure 4

α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors and AMPA scaffold proteins are downregulated in LHON-RGCs. (A) Schematic showing KEGG pathway “Glutamatergic synapse” with the genes downregulated in LHON-RGCs marked with asterisks. (B) ELISA measurement of glutamate secreted by 7-day and 14-day Normal-RGCs and Control-RGCs. Mean concentrations from three independent measurements are shown with standard deviation error bars. (C) Western blot analysis demonstrating expression of GluR1 and GluR2 in day-3, day-7 and day-14 Normal-RGCs and LHON-RGCs. GAPDH used as a loading control. (D) Quantification of Western blot expression levels of GluR1 and GluR2. Means from three independent experiments are shown with standard deviation error bars. ** p < 0.01, * p < 0.05. (E) Immunofluorescent staining of GluR2 in Normal-RGCs and LHON-RGCs. Signal intensity was quantified by ImageJ and shown below. Nuclei stained with DAPI, neurites–by immunostaining of F-actin. (F) Western blot analysis of expression of AMPA receptor-associated scaffold proteins in Normal-RGCs and LHON-RGCs at different stages of maturity. GAPDH used as a loading control.