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Protein–protein interactions between AMPA receptors and their associated scaffold proteins in LHON-RGCs. (A) Western blot analysis of PICK1, Parkin and GRIP1 immunoprecipitates from lysates of 21-day Normal-RGCs and LHON-RGCs (right panel). The aliquots of total lysates were analyzed in parallel (left panel). (B) Quantification of signal intensity in the indicated immunoprecipitates using ImageJ. The signal of protein binding was normalized to the signal of immunoprecipitated protein.
