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. 2019 Jun 15;7(3):98–109.

Figure 3.

Figure 3

Different effects of PPARγ isoforms in LNCaP cells. LNCaP cells were transfected with vector control, PPARγ1, or PPARγ2 expression vectors along with PPRE-luc and pRL SV40 (for B). A. Western blot for expression of PPARγ1 or γ2 in transfected LNCaP cells. B. 24 hours following the treatment of transfected cells with the indicated drugs, relative luciferase activity was measured. PPARγ activity was stimulated by PPARγ agonist pioglitazone (pio), and inhibited by the PPARγ antagonist T0070907 (T007) in LNCaPs expressing either γ1 or γ2 (*P<0.05 difference from pio treated cells). C. The growth of transfected LNCaP cells relative to day 0 was measured. Exogenous expression of γ1 did not affect growth of LNCaP cells; however γ2 overexpression decreased growth of LNCaP cells. Neither was inhibited by T007 (*P<0.05 difference from control cells). D. The growth of transfected LNCaP cells in a soft agar colony forming assay was measured. Exogenous expression of γ1 did not affect growth of LNCaP colonies; however γ2 overexpression decreased growth of LNCaP cells. Neither was inhibited by T007 (*P<0.05 difference from control cells).