Figure 4.

Effects of PPARγ inhibition in LCP cells. A. Western blot for expression of PPARγ in LCP cells, which are LNCaP cells with androgen receptor (AR) and PPARγ1 constitutive expression. B. Cells were transfected with PPRE-luc + pRL SV40 and treated as indicated. The following day, relative luciferase activity was measured. PPARγ activity was stimulated by pio, and inhibited by T007 in LCP cells (*P<0.05 difference from pio treated cells). C. The growth of LCP cells relative to day 0 was measured and was found to be sensitive to T007 (*P<0.05 difference from control cells). D. The growth of LCP cells in a soft agar colony forming assay was measured and was found to be inhibited by T007 (*P<0.05 difference from control cells).