Figure 7.

Different effects of PPARγ isoforms in mPrE cells. Benign mouse prostate epithelial (mPrE) cells were derived from PPARγ-/- mouse prostates and had γ1 or γ2 stably reintroduced [20]. They express either PPARγ1 or γ2, respectively. A. Cells were transfected with PPRE-luc + pRL SV40 and treated as indicated. The following day, relative luciferase activity was measured. PPARγ1 and γ2 activity was stimulated by pio and inhibited by T007 (*P<0.05 difference from pio treated cells). B. The growth of mPrEC cells relative to day 0 was measured. Cells expressing γ1, but not γ2, were sensitive to T007 (*P<0.05 difference from control cells). C. The growth of mPrE cells in a soft agar colony forming assay was measured and it was found that the γ1-expressing cells grew better than the γ2-expressing cells in this assay. mPrEγ growth was inhibited by T007 (*P<0.05 difference from control cells).