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. 2019 Jun 12;8(6):578. doi: 10.3390/cells8060578

Figure 1.

Figure 1

DEAD-box helicase 4 (DDX4) immunohistochemical staining of human ovarian tissue and rat skeletal muscle. (A) DDX4-positive (brown staining) germ cells (oocytes) in a section of ovarian cortical tissue from a 23-year-old woman. Scale bar, 100 µm. (B) DDX4-positive staining in human ovarian tissue sections from two women aged 31 and 34 years. Small cells staining positive (red arrows) but not associated with oocytes or follicular cells; using Abcam 13840 antibody (i), (i) and (iv) and LS-C97782 antibody (iii). When focusing on (B)(iv), it is clear to see the DDX4 staining in the transitory follicles (as expected), in addition to the small putative oogonial stem cell (OSC). Scale bar, 20 µm. (C) Negative control: Adult human ovary; primary antibody omitted; no staining of oocytes or other cells. Scale bar, 100 µm. (D) Rat skeletal muscle negative control using primary antibody LS-C97782; no staining was visualised. Scale bar, 100 µm; ×40 magnification.