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. 2019 Jun 18;8(6):606. doi: 10.3390/cells8060606

Figure 1.

Figure 1

Effect of compound 89 (C89) on female germline stem cell (FGSCs) in vitro. (A) Structure of C89. (B) Cellular morphology of FGSCs treated with various concentrations of C89at 48 h. Bar: 50µm. i: Control (DMSO), ii: 0.125 μM, iii: 0.25 μM, iv: 0.5 μM, v: 1 μM, vi: 2 μM. (C) The amount of various concentrations C89-treated FGSCs. The number of FGSCs was significantly reduced from 0.5to 2µM C89-treated groups compared to control. (D,E) Cellular viability of FGSCs treated with C89 for 24 h (D) or 48 h (E) as detected by Cell Counting Kit 8 (CCK8) assay. FGSCs showed significant differences in cell viabilities between the 0.5, 1, and 2 μM C89-treated groups and control groups (p < 0.01). (F,G) Proliferation of C89-treated FGSCs at 48 h as determined using the 5-ethynyl-2’-deoxyuridine (EdU) staining. Significant differences in proliferation were observed between 0.5, 1, and 2 μM C89-treated groups and the control groups (p < 0.01). Bar: 25 µm. i: Control (DMSO), ii: 0.125 μM, iii: 0.25 μM, iv: 0.5 μM, v: 1 μM, vi: 2 μM. * p < 0.05, ** p < 0.01, *** p < 0.001.