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. 2019 Jun 18;8(6):606. doi: 10.3390/cells8060606

Figure 2.

Figure 2

C89 induces autophagy in FGSCs in vitro. (A–C) Western blot analysis of autophagy marker proteins LC3BII and SQSTM1 in FGSCs treated with C89 or controls. LC3BII expression was significantly higher in C89-treated groups than in control groups (B) (p < 0.01), and SQSTM1 expression was significantly lower in C89-treated groups than in control groups (C) (p < 0.001). (D) Immunofluorescence assay of LC3B puncta in C89-treated FGSCs. The number of LC3B puncta per cell in C89-treated groups was significantly higher than in control groups (p < 0.01). * p < 0.05, ** p < 0.01, *** p < 0.001.