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. 2019 Mar 31;8(2):22. doi: 10.3390/biology8020022

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell survival due to warm anoxia-reoxygenation and the effect of α-tocopherol. The XTT assay revealed that cell survival in mouse RPTECs was decreased in contrast to hamster RPTECs that well tolerated 24 h of anoxia followed by 2 h of reoxygenation. The presence of α-tocopherol (Toc) did not affect the cell viability of hamster RPTECs. However, it significantly enhanced cell viability of mouse RPTECs subjected to anoxia-reoxygenation. These experiments were repeated nine times in triplicate. Error bars correspond to SEM, the asterisk (*) to p < 0.001 when compared to the respective control cells, and the hashtag (#) to p < 0.001 between cells subjected to anoxia-reoxygenation without or with α-tocopherol. Mo stands for mouse, Hm for hamster, ctrl for control, Reox for reoxygenation, and Toc for α-tocopherol.