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. 2019 Mar 31;8(2):22. doi: 10.3390/biology8020022

Figure 4.

Figure 4

Expression of xCT, ferritin H, ferritin L and GPX4 in mouse and hamster RPTECs subjected to anoxia or reoxygenation. In mouse RPTECs, anoxia enhanced the levels of xCT, ferritin H, ferritin L, and GPX4 (panels A, B, C, and D respectively). During reoxygenation, at the time of the increased reactive oxygen species production, xCT, ferritin H, and ferritin L decreased, whereas GPX4 increased (panels A, B, C, and D respectively). In hamster RPTECs, anoxia also increased the levels of xCT, ferritin H, ferritin L, and GPX4 (panels A, B, C, and D respectively). However, reoxygenation raised xCT, ferritin H, and ferritin L further, but lowered GPX4 (panels A, B, C, and D respectively). These experiments were repeated nine times. Error bars correspond to SEM. Except for the asterisk (*) that detected the significance of GPX4 increase in mouse RPTECs in panel (D) and corresponds to a p < 0.05, in all other cases, the asterisk (*) corresponds to p < 0.001 when compared to the respective control cells and the hashtag (#) to p < 0.001 between cells subjected only to anoxia and cells subjected to anoxia followed by reoxygenation. Mo stands for mouse, Hm for hamster, ctrl for control, Anox for anoxia, and Reox for reoxygenation.