Figure 2.

DNA content, glucose consumption and lactate production of spheroids. (a) DNA was extracted from 10 spheroids and measured at days 0, 3, 6 and 9. Blue and red represent polystyrene or gas-permeable conditions, respectively. Broken and solid lines represent normal or hydrogel bead-embedded spheroids. (b) Glucose consumption rate was calculated at day 3 and expressed as fold-change relative to Control condition. (c) Lactate production rate was calculated at day 3 using the same samples as for glucose consumption. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.