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. 2019 Jun 19;20(12):2992. doi: 10.3390/ijms20122992

Figure 2.

Figure 2

Effects of sildenafil and leucine on cAMP concentrations in 3T3L1 cells. cAMP response in 3T3L1 adipocytes treated with (A) sildenafil (1 nM to 10 µM) alone or (B) in combination with Leucine (0.5 mM) for 30 min. Data were calculated and statistically analyzed as a fold change of control and are expressed as means ± SEM (n= 3 to 5, p ≤ 0.05) (absolute values for control means are 3.922 and 3.48 pmol cAMP/mg protein for Sildenafil and Sildenafil-Leucine, respectively). * indicates a significant difference from Control (p < 0.04). (C) Comparison of cAMP response in 3T3L1 adipocytes treated with sildenafil-leucine +/− PDE2 inhibitor (BAY 60-7550, 10 µM) for 30 min. * indicates significant difference between the two curves (p < 0.05).