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. 2019 Jun 19;20(12):2992. doi: 10.3390/ijms20122992

Figure 4.

Figure 4

Protein expression of Phospho (Thr172)-AMPK and EPAC in 3T3L1 adipocytes. (A) P-AMPK and (B) EPAC protein expression in 3T3L1 cells treated with sildenafil (1 nM to 10 µM) in combination with leucine (0.5 mM) for 2 hours. Graphs were calculated from pooled densitometry measurements of two different blots (n = 4) and representative blots are shown. * indicates a significant difference from the control (p < 0.05).