Figure 6.

Change in cAMP and P-AMPK protein expression for treatment with PDE siRNA. HepG2 cells were treated with or without PDE2 siRNA for 48 hours, which was followed by 30-minute treatment with Sildenafil (1 or 10 µM) – Leucine (0.5 mM). (A) Intracellular cAMP concentration was measured. * indicates a significant difference between the cells treated with siRNA as opposed to the ones not treated with PDE2 siRNA (p < 0.02). (B) Phospho (Thr172)-AMPK protein expression was measured. Bar graphs of densitometry measurements and representative blots are shown (n = 3).