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. 2019 Jun 6;11(6):784. doi: 10.3390/cancers11060784

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of p75NTR and signaling effectors involved in locomotion of castration-resistant prostate cancer (CRPC) cells. C4-2B, DU145 and PC3 cells were used. In A, lysates from the indicated cell lines were prepared and proteins were analyzed by western blot, using the antibodies against p75NTR. In B, lysates from the indicated cell lines were prepared and proteins were analyzed by Western blot, using the antibodies against the indicated proteins. Filters were re-probed using anti tubulin antibody, as a loading control. Western blots in A and B are representative of three different experiments. Expression levels of proteins were analyzed by densitometry analysis, using NIH Image J Software. The ratio between Filamin A/tubulin (C), FAK/tubulin (D) and Paxillin/tubulin (E) was evaluated. Results were expressed as relative ratio. Means and SEMs are shown, n represents the number of the experiments.