Figure 4.

In vitro coculture with M2-macrophages does not differentially affect growth of PancTu-I shCtrl and shTR2 cells. PancTu-I shCtrl or PancTu-I shTR2 cells were indirectly cocultured in absence (mono) or presence of M2-macrophages (+ M2-macrophages) for one week. After coculture, (A) vital cell numbers and (B) Ki67 status were analyzed. Vital cell numbers were obtained by counting living cells in a Neubauer counting chamber. Ki67 status was determined by immunocytochemical Ki67 staining. Proportion of Ki67+ cells was normalized to monocultured PancTu-I shCtrl cells. Data represent the mean ± SEM of 4–5 independent experiments.