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. 2019 May 29;11(6):745. doi: 10.3390/cancers11060745

Figure 7.

Figure 7

HSC-mediated growth suppression of PancTu-I shTR2 cells is CXCL-8/IL-8 dependent. Supernatants of PancTu-I shCtrl and PancTu-I shTR2 cells cocultured with HSC or HMF were analyzed for human CXCL-8/IL-8 levels by LEGENDplex multiplex analysis. (A) Detected CXCL-8/IL-8 concentrations were normalized to vital cell counts of corresponding PancTu-I cells. For blocking experiments, PancTu-I shCtrl and PancTu-I shTR2 cells were indirectly cocultured in the presence of HSC and treated with either 10 µg/mL control IgG1 or anti-CXCL-8/IL-8 antibody. After 6 days of coculture, (B) vital cell count and (C) the proportion of Ki67+ cells were determined. The percentage of proliferating cells was determined by immunofluorescence Ki67-Alexa-488 staining. Data are normalized to control group shCtrl + HSC + control IgG1. (D) Representative images of immunofluorescence Ki67 staining are shown. Scale Bars 100 µm. Data represent the mean ± SEM of 4–5 independent experiments; * = p < 0.05.