Table 1.

Univariate and multivariate survival analysis in 192 ovarian cancer patients. RANK/RANKL/OPG cut-off was determined by the Youden Index.

Univariate Survival Analysis in 192 Ovarian Cancer Patients
Variable		Progression Free Survival			Overall Survival
		No. Patients	Median, Years	p Value	No. Patients	Median, Years	p Value
		(Relapsed/Total)	(95% CI)		(Died/Total)	(95% CI)
Age (median)	≤50.0 yrs.	21/32	2.13 (4.03–9.17)	0.762	15/32	9.15 (9.49–17.69)	0.019
	>50.0 yrs.	92/160	2.00 (6.71–10.11)		105/160	3.9 (7.00–10.04)
FIGO stage	I/II	10/50	n.r. (13.63–18.83)	<0.001	18/50	n.r. (12.23–18.08)	<0.001
	III/IV	103/142	1.47 (4.03–6.95)		102/142	3.80 (6.07–9.26)
Tumour grade	1–2	54/99	2.06 (6.98–11.34)	0.249	57/99	6.76 (9.09–13.78)	0.07
	3	57/91	1.98 (5.14–9.00)		61/91	3.71 (5.57–8.92)
Residual disease after surgery	no	38/96	n.r. (10.54–15.10)	<0.001	35/96	n.r. (11.98–16.49)	<0.001
	yes	71/90	1.25 (2.25–5.02)		81/90	2.55 (3.74–6.62)
Histology	HGSOC	81/122	1.81 (4.28–7.41)	0.003	88/122	3.70 (6.04–9.57)	0.001
	others	30/66	5.98 (9.31–14.68)		29/66	11.06 (10.31–15.68)
RANKL mRNA expression	low	58/115	3.56 (1.81–5.31)	0.01	62/115	8.76 (5.98–11.55)	0.005
	high	55/77	1.68 (1.21–2.16)		58/77	3.62 (2.51–4.74)
RANK mRNA expression	low	24/48	3.56 (0.89–6.24)	0.117	26/48	9.27 (1.97–16.56)	0.03
	high	89/144	1.97 (1.54–2.40		94/144	3.71 (1.85–5.57)
OPG mRNA expression	low	101/177	2.06 (1.33–2.80)	0.135	107/177	5.80 (3.94–7.66)	0.154
	high	12/15	1.46 (1.16–1.76)		13/15	3.29 (1.87–4.70)
Note: The significance level (p) was determined by log-rank test. RANK/RANKL/OPG cut-off was determined by the Youden Index. Abbreviations: CI, confidence interval; HGSOC, high grade serous ovarian cancer; n.r., not reached.
Multivariate Survival Analysis in 192 Ovarian Cancer Patients
Variable		Progression Free Survival			Overall Survival
		RR	(95 CI)	p Value	RR	(95 CI)	p Value
Age	≤50.0 yrs >	1.19	(0.73–1.96)	0.489	1.54	(0.87–2.75)	0.134
FIGO stage	I/II vs. III/IV	3.57	(1.76–7.27)	<0.001	1.05	(0.58–1.90)	0.872
Tumour grade	1/2 vs. 3	1.09	(0.73–1.62)	0.682	1.08	(0.73–1.58)	0.705
Residual disease after surgery	no vs. Yes	2.01	(1.28–3.17)	0.003	4.96	(1.78–4.62)	<0.001
Histology	HGSOC vs. others	0.87	(0.55–1.36)	0.533	0.72	(0.46–1.12)	0.14
RANKL mRNA expression	low vs. high	1.42	(0.97–2.10)	0.017	1.7	(1.16–2.50)	0.007
RANK mRNA expression	low vs. high	1.19	(0.72–1.96)	0.502	1.36	(0.85–2.19)	0.202
OPG mRNA expression	low vs. high	1.11	(0.59–2.07)	0.749	1.25	(0.68–2.29)	0.466
Note: The significance level (p) was determined by Cox regression. RANK/RANKL/OPG cut-off was determined by the Youden Index. Abbreviations: CI, confidence interval; HGSOC, high grade serous ovarian cancer; RR, relative risk.