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Permeabilizing effect of arenicin-1 variants on E. coli ML35p outer and cytoplasmic membranes. The incubation wells contained 10mM sodium phosphate buffer, 100 mM NaCl, 2.5 × 107 colony-forming units (CFU) of washed, stationary-phase E. coli ML35p, and the peptides of interest at a concentration equivalent to 2 × MIC, or an equivalent volume of acidified water (controls).
