Table 2.
Carotenoid profiles based on the LC-MS data of the extract of the nutrient-depleted conditions from Coelastrum sp. TISTR 9501RE (a representative set of data from three experiments).
| Entry | Identity * | Retention Time ** | Proposed Formula | Precursor Mass | Found at Mass | Mass Error (ppm) |
|---|---|---|---|---|---|---|
| 1 | All-trans-astaxanthin | 16.22 | C40H52O4 | 597.3938 | 597.3935 | −0.53 |
| 2 | Violaxanthin isomer | 17.28 | C40H56O4 | 601.4251 | 601.4251 | −0.04 |
| 3 | All-trans-lutein | 19.33 | C40H56O2 | 569.4353 | 569.4327 | −4.54 |
| 4 | Chlorophyll b | 19.33 | C55H70N4O6Mg | 907.5219 | 907.5218 | −0.03 |
| 5 | All-trans-Canthaxanthin | 23.46 | C40H52O2 | 565.4040 | 565.4035 | −0.87 |
| 6 | Chlorophyll a | 25.65 | C55H72N4O5Mg | 893.5426 | 893.5425 | −0.12 |
| 7 | AME C18:4 isomer | 30.67 | C58H78O5 | 855.5922 | 855.5918 | −0.51 |
| 8 | AME C18:1 isomer | 37.69 | C58H84O5 | 861.6392 | 861.6392 | 0.02 |
| 9 | Chlorophyll b epimer | 38.64 | C55H70N4O6Mg | 907.5220 | 907.5329 | 12.2 |
| 10 | AME C18:2 isomer | 41.77 | C58H82O5 | 859.6235 | 859.6231 | −0.49 |
| 11 | Chlorophyll a epimer | 43.00 | C55H72N4O5Mg | 893.5426 | 893.5507 | 9.04 |
* AME = Astaxanthin monoester. C18:n indicates an unsaturated fatty acyl part, with n being the number of double bonds in the molecule. ** Data from the HPLC-PDA run.