Figure 5.

Effect of free DHA and DHA-RV-SLNs on ROS production in HaCaT keratinocytes treated with SDS or TNF-α. The cells were treated with free DHA or DHA-RV-SLNs (10 or 30 µM) in the absence or in the presence of 50 µg/mL SDS (A) or 50 ng/mL TNF-α (B) for 24 h, in basal conditions (left panels) or in the presence of 100 µM H₂O₂ as a pro-oxidant stimulus (right panels). Data are the means ± SD of three different experiments. Values not sharing the same superscript letter are significantly different (p < 0.05, one-way ANOVA followed by Tukey’s test).