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. 2019 Jun 11;8(6):573. doi: 10.3390/cells8060573

Figure 4.

Figure 4

FOS is a direct target gene of miR-501-3p. (A) The sequence of mature miR-501-3p of different species. (B) The alignment result of FOS 3′UTR sequence from different species. Red: the complete complementary sequence of the FOS 3′UTR. (C) The complementary pairing of mmu-miR-501-3p with the targeted gene FOS 3′UTR or mutated UTR. (D) FOS mRNA level was negatively correlated with miR-501-3p level. (E) FOS protein level was negatively correlated with miR-501-3p level. (F) miR-501-3p inhibited the pmirGLO-FOS 3′ UTR vector luciferase activity. Luciferase reporters were transfected into HEK-293T cells with either miR-501-3p mimics or control. Luciferase activity was assayed 24 h after transfection. ** p < 0.01. *** p < 0.001. The results are presented as mean ± S.E.M. of three replicates for each group.