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. 2019 Jun 11;8(6):573. doi: 10.3390/cells8060573

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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miR-501-3p formed a feedback loop with FOS, MDFI, and MyoD. (A) Schematic representation of MyoD binds at the upstream of miR-501-3p in the genome, and FOS binds at the upstream of MDFI in the genome. (B) ChIP experiments were used to verify that FOS and MyoD bound to the promoter regions of MDFI and Clcn5, respectively. (C) Luciferase reporters show FOS bound to the MDFI promoter region. (D) Luciferase reporters show MyoD bound to the Clcn5 promoter region. (E) QPCR results indicate that FOS inhibited the expression of MDFI and MyoD increased the expression of Clcn5 and miR-501-3p. (F) A feedback loop formed by miR-501-3p, FOS, MDFI, and MyoD to regulate myogenic differentiation. **, p < 0.01; ***, p < 0.001. The results are presented as mean ± S.E.M. of three replicates for each group.