Table 3.
The candidate differential targets of cold-responsive miRNAs in Populus simonii × P. nigra.
| Target ID | Annotation | miRNA ID | Predicted Binding Site | Negative Regulation |
|---|---|---|---|---|
| Cluster-16493.31604 | PREDICTED: coatomer subunit alpha-1-like isoform X1 | novel_48 | miRNA: 1 TGTGGGAATGAACATTATGAG 21 | : | || :|||||| | | : : | : || : Target: s312 ATACCTTTACTTGTGGTGCTT 292 |
yes |
| Cluster-16493.38601 | Putative EG45-like domain containing protein 1 | ptc-miR167e | miRNA: 1 TGAAGCTGCCAGCATGAT-CTG 21 |||| ||| : ||||| | *|| | **|| Target: 259 ACTTCGATGGTCGTCCTACAAC 238 |
no |
| Cluster-16493.31604 | PREDICTED: coatomer subunit alpha-1-like isoform X1 | ptc-miR168a-5p | miRNA: 1 TCGCTTGGTGCAGGTCGGGAA 21 |* ||||| | | : ||: : ||| | | || Target: 3706 ACCGAACCATGTTTAGCCCTT 3686 |
no |
| Cluster-16493.38601 | Putative EG45-like domain containing protein 1 | ptc-miR167f-5p | miRNA: 1 TGAAGCTGCCAGCATGATCTT 21 |||||| | : |||| | | *||| *|| Target: 259 ACTTCGATGGTCGTCCTACAA 239 |
no |
| Cluster-16493.38601 | Putative EG45-like domain containing protein 1 | ptc-miR167a | miRNA: 1 TGAAGCTGCCAGCATGATCTA 21 ||||| || : ||| ||| * |||* |* Target: 259 ACTTCGATGGTCGTCCTACAA 239 |
no |
| Cluster-16493.40057 | LINE-1 retrotransposable element ORF2 protein | ptc-miR396e-3p | miRNA: 1 CTCAAGAAAGCTGTGGGAGA 20 * : *|| |||| |||||| : | | | | Target: 2923 CGCTTCTTTCGACACTCTCT 2904 |
no |
| Cluster-16493.31604 | PREDICTED: coatomer subunit alpha-1-like isoform X1 | ptc-miR482a.1 | miRNA: 1 CCTACTCCTCCCATTCC 17 ||||* | |* | || || |: || Target: 1901 GGATAAGAAGGGTAGGG 1885 |
no |
| Cluster-16493.26584 | Calcium-transporting ATPase 4 | ptc-miR482a.1 | miRNA: 1 CCTACTCCTCCCATTCC 17 *|| *| || || |* |||||| Target: 4880 TGAAGAGGAGTGTAAGG 4864 |
no |
| Cluster-16493.41325 | Polyphenol oxidase, chloroplastic | ptc-miR1444d | miRNA: 1 CGAACGTTGACCGAATGT-GAA 21 || ||||| : |||||||||| * | * | Target: 85 GCTTGCAGCTGGCTTACACCCT 64 |
yes |
| Cluster-16493.29872 | Basic leucine zipper 63 | ptc-miR172a | miRNA: 1 AGAATCTTGATGATGCTGCAT 21 | : || | |||||| |* |||| |* * | Target: 1241 TTTTAGAACTACGACGACCAA 1221 |
yes |
| Cluster-16493.41106 | Putative SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 2 | ptc-miR172a | miRNA: 1 AGAATCTTGATGATGCTGCAT 21 | || || |||| | |*|*| || * ||| Target: 3710 TCTTAGAACTAGTCCGA-GTA 3691 |
no |
| Cluster-16493.44708 | Heat shock protein 90-1 | ptc-miR319i | miRNA: 1 TTGGGCTGAAGGGAGCTCCC 20 ||| * ||: | | || : || || ||| * Target: 890 AACGCGGCTTCTCTCGAGGT 871 |
yes |
| Cluster-16493.49274 | 18.2 kDa class I heat shock family protein | ptc-miR7812 | miRNA: 1 CTGTTATGAATTGATGGAGTG 21 * ||* ||| |||| || |||||*|| Target: 305 AACTATACTTAACTACCT-AC 286 |
yes |
| Cluster-16493.45983 | endo-glucanase 2 family protein | ptc-miR6462e | miRNA: 1 TCTTATGCGTTTTTGTCTCT 20 |||| || ||| ||| : : : | |* *| Target: 590 AGAATACGCAAAGGTAG-TA 572 |
yes |