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Lactoferricin (LFcin) interacts with polySia in a chain-length-dependent manner. Bovine LFcin (10 µg; molecular weight of 3 kDa) was incubated with polySia (5 µg) using groups with different degree of polymerization (DP). Subsequently, the samples were separated using native agarose gel electrophoresis. As positive control, lactoferrin was used (10µg). Proteins were stained with Coomassie blue.
